Figure 5From: EBNA1 binding and epigenetic regulation of gastrokine tumor suppressor genes in gastric carcinoma cellsEBV reinforces the repression of GKN1 and GKN2 gene expression by DNA methylation. (A) AGS cells were treated with 10 μM Aza, or 1 mM NaB and 20 ng/mL TPA for 48 hours and analyzed by RT-PCR for GKN1 or GKN2 expression level, compared with untreated AGS. (B) AGS cells or AGS-EBV cells were treated or untreated with 10 μM Aza for 48 hours, then assayed for GKN1, GKN2 mRNA, or EBNA1 mRNA levels. (C) MeDIP assay of AGS or AGS-EBV cells treated or untreated with 10 μM Aza for 48 hours at different DNA regions of GKN1 and GKN2 loci. (D) MeDIP assay of AGS or AGS-EBV cells treated or untreated with 10 μM Aza for 48 hrs at cellular regions for alpha globin-2, GAPDH, CDC7, FOXP2, HDAC3, or MAP3KIP2. (E) Genome position of primers used for GKN1-GKN2 ChIP and MeDIP assays. * indicates p < .05, ** indicates p < .005. Error bars indicated sdm for n = 3.Back to article page