Skip to main content

Table 2 The in silico XbaI restriction enzyme digestion of ATI-2 DNA sequences

From: Molecular characterization and phylogenetics of Fennoscandian cowpox virus isolates based on the p4c and atip genes

Virus strain Amplicon size (bp) XbaI digest fragments (bp)b RFLP profile group
CPXV-BR 1673 645, 515, 346, 100, 67 1
CPXV-No-H1 1601 645, 543, 346, 67 2
CPXV-No-F1 1601 645, 543, 346, 67 2
CPXV-No-F2 1601 645, 543, 346, 67 2
CPXV-Swe-H1 1601 645, 443, 346, 100, 67 3
CPXV-Swe-H2 1601 645, 443, 346, 100, 67 3
CPXV-FIN/T2000 1672 645, 345, 299, 144, 100, 72, 67 4
CPXV-No-H2 1127 482, 343, 151, 151 5
ECTV-MOS 1220 575, 343, 151,151 6
  1. bThe ATI-2 PCR products generated with ATI-2 primer pairs as described in Methods were sequenced and the exact sizes of the amplicons were derived from the sequences while the exact sizes of the restriction fragments were obtained by in silico digestion of the ATI-2 DNA sequences with XbaI as implemented in Chromas Pro 1.7.5 (http://technelysium.com.au/?page_id=27).