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Table 2 The in silico XbaI restriction enzyme digestion of ATI-2 DNA sequences

From: Molecular characterization and phylogenetics of Fennoscandian cowpox virus isolates based on the p4c and atip genes

Virus strain

Amplicon size (bp)

XbaI digest fragments (bp)b

RFLP profile group

CPXV-BR

1673

645, 515, 346, 100, 67

1

CPXV-No-H1

1601

645, 543, 346, 67

2

CPXV-No-F1

1601

645, 543, 346, 67

2

CPXV-No-F2

1601

645, 543, 346, 67

2

CPXV-Swe-H1

1601

645, 443, 346, 100, 67

3

CPXV-Swe-H2

1601

645, 443, 346, 100, 67

3

CPXV-FIN/T2000

1672

645, 345, 299, 144, 100, 72, 67

4

CPXV-No-H2

1127

482, 343, 151, 151

5

ECTV-MOS

1220

575, 343, 151,151

6

  1. bThe ATI-2 PCR products generated with ATI-2 primer pairs as described in Methods were sequenced and the exact sizes of the amplicons were derived from the sequences while the exact sizes of the restriction fragments were obtained by in silico digestion of the ATI-2 DNA sequences with XbaI as implemented in Chromas Pro 1.7.5 (http://technelysium.com.au/?page_id=27).
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Virology Journal

ISSN: 1743-422X