Figure 4

Inhibition of mTOR alters late lytic replication in EBV-positive cells. AGS-BDneo (A), Raji (B), and EBfaV-GFP (C) cells were left untreated or treated with 5 nM rapamycin for 24 hr prior to induction of lytic replication, as indicated. 48 hr post-infection cells were immunostained for BMRF1 or VCA, and the staining was analyzed by flow cytometry. The percent positive cells is presented. D. EBfaV-GFP cells were left untreated or treated with 5 nM rapamycin for 24 hr prior to induction of lytic replication, as indicated. 2 days post-induction the media from each condition was filtered and placed on Raji cells for 2 days. The number of resulting infected Raji cells was quantified, as judged by GFP, and the percent of infection presented. * = P < 0.05 relative to uninduced, untreated control cells; # = P < 0.05 relative to induced, untreated cells.