Figure 2

Effects of disruption of microfilaments on ISKNV infection. (A) MFF-1 cells were treated with 0.2 μg/ml (2) or 0.5 μg/ml cyto B (3), Mock- treated MFF-1 cells were used as control (1). (B) MFF-1 cells were treated with 2 μM (5) or 5 μM cyto D (6), Mock- treated MFF-1 cells were used as control (4). (C) MFF-1 cells were treated with 2 μM (8) or 5 μM lat A (9), Mock- treated MFF-1 cells were used as control (7) (D) Mock-treated and uninfected MFF-1 cells were used as negative control. Numbers at the bottom right of each panel refer to the percentages of infected cells per field and represent the mean of at least four fields of cells from each of three independent experiments. The ISKNV ORF101L expression of infected cells was detected by indirect immunofluorescence (green).