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Figure 3 | Virology Journal

Figure 3

From: Development of simple and rapid assay to detect viral RNA of tick-borne encephalitis virus by reverse transcription-loop-mediated isothermal amplification

Figure 3

Detection of TBEV gene from a mixture of TBEV and tick homogenates by TBEV RT-LAMP assay. (A) Real-time kinetics monitored by the turbidity of RT-LAMP products after using 10 ng of RNA extracted from each mixture of TBEV and pooled tick homogenates. (B) Visual detection of color change to cloudy yellow in the RT-LAMP products. The reaction was repeated three times and a representative result is shown. RNA was extracted from 10 mg of ticks containing TBEV Oshima strain of different concentrations between 10-1 to 106 pfu. Approximately 10 μg of RNA was recovered from one sample.

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Virology Journal

ISSN: 1743-422X