Detection of TBEV gene from a mixture of TBEV and tick homogenates by TBEV RT-LAMP assay. (A) Real-time kinetics monitored by the turbidity of RT-LAMP products after using 10 ng of RNA extracted from each mixture of TBEV and pooled tick homogenates. (B) Visual detection of color change to cloudy yellow in the RT-LAMP products. The reaction was repeated three times and a representative result is shown. RNA was extracted from 10 mg of ticks containing TBEV Oshima strain of different concentrations between 10-1 to 106 pfu. Approximately 10 μg of RNA was recovered from one sample.