Figure 1

A. Schematic representation of the SFV expression vectors. SP6 RNA polymerase promoter for transcription in vitro is shown by the filled arrow. Sequences encoding 1-48preS/S variants are placed under the control of SFV 26S subgenomic promoter (empty arrow) and expression is directed by SFV replicase. The filled triangle denotes the modified myristic acid attachment site, where Gly2 was replaced with Ala or Ser in L deletion variants G2A 1–48preS/S and G2S 1-48preS/S. The space between the regions encoding aa 1–48 of preS1 and S/ayw2 denotes the “spacer” encoding aa LEGGSGG. B. Schematic representation of L protein deletion variants consisting of the first 48 aa of preS1 fused to the N-terminus of the S domain showing the myristoylation and potential glycosylation sites.