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Table 1 Primers used in plasmid construction and semi-quantitative RT-PCR

From: The expression of interleukin-32 is activated by human cytomegalovirus infection and down regulated by hcmv-miR-UL112-1

Products

Primer sequences

miR-UL112-1

F: 5′-CGCGGATCC TCAGGTACTCGCAGGTGTGC-3′

 

R: 5′-CCCAAGCTT GTTGCCTGGACGCCTGGGCGCGA-3′

IL-32UTR

F: 5′-GGACTAGT AGATACTGACACCACCTTTGCCCT-3′

 

R: 5′-CCCAAGCTT CATGGTATCTCCCCTGCCAG-3′

IL-32MUT

F: ACCTTTGCCCTCCCCGaaACCGCGCACCCACCCTGA

 

R: TCAGGGTGGGTGCGCGGTttCGGGGAGGGCAAAGGT

IL-32

F: 5′-CATGAATTCCATGCTTCCCGAAGG-3′

 

R:5′-CTACTCGAGGTATCTTCATTTTGAGGATTG-3′

β-actin

F: 5′-CTCCATCCTGGCCTCGCTGT-3′

 

R: 5′-GCTGTCACCTTCACCGTTCC-3′

  1. Note: sequences recognized by restriction en donuclases are down lined.