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Table 1 Primers used in plasmid construction and semi-quantitative RT-PCR

From: The expression of interleukin-32 is activated by human cytomegalovirus infection and down regulated by hcmv-miR-UL112-1

Products Primer sequences
miR-UL112-1 F: 5-CGCGGATCC TCAGGTACTCGCAGGTGTGC-3
  R: 5-CCCAAGCTT GTTGCCTGGACGCCTGGGCGCGA-3
IL-32UTR F: 5-GGACTAGT AGATACTGACACCACCTTTGCCCT-3
  R: 5-CCCAAGCTT CATGGTATCTCCCCTGCCAG-3
IL-32MUT F: ACCTTTGCCCTCCCCGaaACCGCGCACCCACCCTGA
  R: TCAGGGTGGGTGCGCGGTttCGGGGAGGGCAAAGGT
IL-32 F: 5-CATGAATTCCATGCTTCCCGAAGG-3
  R:5-CTACTCGAGGTATCTTCATTTTGAGGATTG-3
β-actin F: 5-CTCCATCCTGGCCTCGCTGT-3
  R: 5-GCTGTCACCTTCACCGTTCC-3
  1. Note: sequences recognized by restriction en donuclases are down lined.