Figure 1

Expression of HA2 or Ag85A in HEK293 cells. (A) Expression of HA2 or Ag85A in HEK293 cells. HEK293 cells were transfected with plasmids encoding individual HA2 or Ag85A proteins or the Ag85A-HA2 fusion protein, and expression was evaluated by GFP fluorescence. Shown are cells transfected with pEGFP/HA2 (a, b), pEGFP/Ag85A (c, d), pEGFP/C2 (e, f), pEGFP/Ag85A-HA2 (g, h) or untransfected cells (i, j). Fluorescent images (a, c, e, g, i) and phase contrast images (b, d, f, h, j) are shown. (B) Expression was evaluated by RT-PCR. (7) cells transfected with pEGFP/ HA2; (3,6,9) Trans2K Plus DNA Marker; (2,5,8) cells transfected with pEGFP-C2; (4) cells transfected with pEGFP/Ag85A; (1) cells transfected with pEGFP/ Ag85A-HA2. Fragments of approximately 1500 bp (1), 750 bp (4, 7) and 500 bp (1, 2, 4, 5, 7 and 8) were separated by 2% agarose gel electrophoresis following RT-PCR. GADPH: Fragments of approximately 500 bp; pEGFP/Ag85A: Fragments of approximately 750 bp; pEGFP/ HA2: Fragments of approximately 750 bp; pEGFP/ Ag85A-HA2: Fragments of approximately 1500 bp. (C) Expression was evaluated by Western blotting. (a) cells transfected with pEGFP/ Ag85A-HA2; (b) cells transfected with pEGFP-C2; (c) cells transfected with PBS; (d) cells transfected with pEGFP/ HA2; (e) cells transfected with pEGFP/Ag85A.