Table 1 List of primers used in both the 1 st and 2 nd rounds of PCR in this study
From: Detection of novel viruses in porcine fecal samples from China
Virus | Targeted contig/read | Targeted region | Primer name | PCR round | Primer sequence | Size (bp) |
|---|---|---|---|---|---|---|
Astrovirus | Contig 15 | Astroviruses ORF1a region | Set A-F | 1st | 5′- STTRCCWTGGSTYTGGGAGAT −3′ | 402 |
Set A-R | 5′- ATMAGCCTCCAGCAGAAGCA −3′ | |||||
Contig 3 | 2ndA-F | 2nd | 5′- ACTGCYTCTACYTWGCAGCAG −3′ | 199 | ||
2ndA-R | 5′- ACATCWGCCARGAACATACC −3′ | |||||
Contig 14 | Astroviruses ORF1a region | Set B-F | 1st | 5′- AGGCTAAACCTCAAGTTAG −3′ | 360 | |
Set B-R | 5′- CCTACAGGAAAAGTTACTC −3′ | |||||
2ndB-F | 2nd | 5′- CACGGATCTCAATGACATGACC −3′ | 316 | |||
2ndB-R | 5′- GCTATTGCACCTATTCAGAC −3′ | |||||
Bocavirus | Contig 5 | Bocaviruses NS1 region | Set C-F | 1st | 5′- TCTGCCTSAGGTSRGTGAGAA −3′ | 224 |
Set C-R | 5′- KATGCATCTCGAGMAYCTCKT −3′ | |||||
Contig 18 | 2ndC-F | 2nd | 5′- GAGAAYTCKCTGGCTAGACAGA −3′ | 185 | ||
2ndC-R | 5′- AYCTCKTCKAGCGTYCGAGA −3′ | |||||
Contig 19 | Bocaviruses NS1 region | Set D-F | 1st | 5′- TCTCTAGCTAGCGAGTTCTC −3′ | 206 | |
Set D-R | 5′- TAGATGAGGTGCTCGAGATGCA −3′ | |||||
2ndD-F | 2nd | 5′- ACCTCATCTAGCGTTCGAGA −3′ | 159 | |||
2ndD-R | 5′- GCCTGCTAAGATCACCAAA −3′ | |||||
LV-like virus | Read 12 | LV-like virus 2C region | Set E-F | 1st | 5′- CTCTTCTGCTATGGAACTGCT −3′ | 452 |
Set E-R | 5′- TTGCCGAAGTATCAGGCTTC −3′ | |||||
2ndE-R | 2nd | 5′- CTGCTTGGCAATGATGTGTCGA −3′ | 404 | |||
2ndE-F | 5′- ACAAACGTGTGCAGTTTCTGG −3′ |