Table 1 Primer sequences
From: Molecular investigation of the 7.2 kb RNA of murine cytomegalovirus
Target | Purpose | Sequence 5′ to 3′ |
|---|---|---|
7.2 kb Intron (a and a’) | qPCR | Fwd: GAGTCAGTTCTAACCCATCACG |
Rev: AGCTCGAAAGTTGAACGGG | ||
Probe: ACGAACGGGTAAAACGGGTAAGGG | ||
Exon2 (b and b’) | qPCR | Fwd: CCACTACCTCTCGATGACAAC |
Rev: AGCGAATTCTAGCGTTACCG | ||
Probe: CGGAGCCTGCGACTTGTCTGC | ||
Spliced mRNA (c and c’) | qPCR | Fwd: TTATCACACCTGAGCGAACG |
Rev: GCAGAGTTCGATGTGTCCG | ||
Probe: AGGATGCGAGATGGCGACGG | ||
M54 | qPCR | Fwd: AACATATCCCTGCCGATCTTG |
Rev: CAACGCTTTCTACGGTTTCAC | ||
Probe: ATGCTCCCGTGTCTCCCCATC | ||
GAPDH | qPCR | Fwd: GTGGAGTCATACTGGAACATGTAG |
Rev: AATGGTGAAGGTCGGTGTG | ||
Probe: TGCAAATGGCAGCCCTGGTG | ||
Actin B | qPCR | Fwd: CTTGATCTTCATGGTGCTAGGAG |
Rev: CGTTGACATCCGTAAAGACCT | ||
Probe: ACCATGTACCCAGGCATTGCTGA | ||
18 srRNA | qPCR | Fwd: GTTGATTAAGTCCCTGCCCTT |
Rev: ATAGTCAAGTTCGACCGTCTTC | ||
Probe: ACCGATTGGATGGTTTAGTGAGGCC | ||
Exon1 | Primer extension | 497:GGCCTTCGGGACGCCGTCACCTCCGCCGCCGC |
Exon2 | 3′RACE | 541: GATCGTTGTCGTCTCTGTCGTGTT |
3′RACE | 542: TGTCATCGAGAGGTAGTGGAGGAT | |
5′RACE | 571: ATCCTCCACTACCTCTCGATGACA | |
5′RACE | 572: AACACGACAGAGACGACAACGATC | |
Northern | 253: GTCGACATGGCGACGGCGAGCCAGCAA | |
Blot | 263: GCGGCCGCGTCTACCACCTCGACCACGATT | |
Probe | ||
7.2 kb Intron | Northern | 262: CTCCAATCGGCCTAGGAATCCTGGCTAGGT |
Blot | 263: AGCAACACGATGCTCTGTGTCGTCGGTCGG | |
Probe | ||
M112/113 | pGL3 Construct | 459: ACGAAGGTCTTTTCACCGGT |
435: ACCATCTGCTAGGCGGGTCC | ||
7.2PR1 | pGL3 Construct | 28: AGATAGCGCGGCGTCCGTCG |
349: CTGAGAGCTCCGGGCCTTCGG | ||
7.2PR2 | pGL3 Construct | 133: AAAAGAAAGTCCGTGACCGGGTCG |
349: CTGAGAGCTCCGGGCCTTCGG |