Figure 1
From: Molecular investigation of the 7.2 kb RNA of murine cytomegalovirus
Expression kinetics of intron locus transcripts. Total RNA was harvested from infected mouse fibroblasts (MOI = 1.0) at the indicated times and analyzed by northern blot analysis using radio-labeled, antisense RNA probes specific for (A) the intron or (B) exon 2 of the spliced mRNA. To determine the kinetics of intron locus expression, cells were pre-treated with either PAA or CHX for 1 hour prior to infection. Total RNA was prepared at the indicated times.