Detection of HPV and EBV antigen by Immunohistochemistry. Immunohistochemistry analysis was performed on sections of cervix tissue samples embedded in paraffin. HPV and EBV antigens were detected by peroxidase mediated DAB-staining (Brown). All samples were counterstained with hematoxylin-eosin and then observed under a light microscope (original magnification 200x). Positive response is marked with a white arrow. A: Squamous-cell carcinoma observed by heamatoxylin-eosine staining. B: Squamous carcinoma cells positive to EBNA-1 revealed by monoclonal anti-EBNA-1 antibody (OT-1). C: Squamous carcinoma cells positive to LMP-1 revealed by monoclonal anti- LMP-1 antibody (S12). D: Cytoplasmic and nuclear HPV staining with monoclonal antibodies (K1H8), which recognize HPV type 6, 11, 16, 18, 31, 33, 42, 51, 52, 56 and 58. E: Squamous-cell carcinoma negative to EBNA-1. F: Squamous-cell carcinoma negative to LMP-1. Weak staining of rare lymphocytes, the tumor cells are negative for LMP1.