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Table 4 Evaluation of the analytical sensitivity of the SBV-S3 assay using an SBV-S3 standard

From: Development of a pan-Simbu real-time reverse transcriptase PCR for the detection of Simbu serogroup viruses and comparison with SBV diagnostic PCR systems

 

Viral genome

Internal control

Concentration

Single

Duplex IC2

Duplex β-actin

Duplex IC2

Duplex β-actin

2 × 106

16.3

16.6

16.5

26.7

29.4

2 × 105

19.5

19.8

19.8

26.2

28.5

2 × 104

23.0

23.1

23.3

26.1

28.3

2 × 103

26.3

26.4

26.5

26.2

28.2

2 × 102

29.6

29.8

30.0

26.2

28.3

2 × 101

32.5

32.7

33.6

26.3

28.4

2 × 100

34.4

34.8

34.6

26.4

28.5

2 × 10-1

37.6

39.3*

neg.

26.4

28.7

  1. concentration in copies/μl; single: SBV-S3 single-target assay; duplex IC2: SBV-S3 duplex assay based on IC2 RNA; duplex β-actin: SBV-S3 duplex assay with co-amplification of beta-actin gene; *only one of the duplicates positive; neg.: negative result; columns 2–6 represent mean Cq-values from duplicates.
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Virology Journal

ISSN: 1743-422X