Figure 4

Mapping of the crucial region of SKP1^Maizeinvolved in the P7 -2- SKP1^Maizeinteraction. (A) Two types of SKP1 proteins. Both of the two types have two conserved domains (Skp1_POZ and Skp1) and two variable regions. Type II proteins have an additional C-terminal region. (B) Schematic representation of SKP1^Maize and its truncations in the study. The full-length SKP1^Maize (spanning residues 1 to 176) and its truncations are indicated by pink bars while the deleted regions by dashed lines. The numbers denote the amino acid positions of the proteins. The ability of different SKP1^Maize truncations to interact with P7-2 is indicated on the right (+, positive; -, negative). (C) The interaction between P7-2 and different SKP1 truncations in YTH assays. Yeast colonies expressing AD-SKP1^97-176 with BD-P7-2 were able to grow on the selective medium and turned slight blue. No growth was observed in the yeast expressing BD-P7-2 with AD-SKP1^1-67, AD-SKP1^1-101 or AD-SKP1^1-128.