Detection of lamivudine-resistant variants and mutations related to reduced antigenicity of HBsAg in individuals from the cities of Santos and São Paulo, Brazil

Table 1 Mutations within the pol and S genes related to antiviral resistance, immune escape and diagnostic failure

ID	Subject	Genotype	Mutations in combination in both POL/S genes	Other mutations in POL and S gene	Impact of the S gene mutations
35	BD	D	-	rtL80F, sI195T, sM133L, rtT128P	sI195T- Diagnostic failure and vaccine/HBIg therapy escape [11]
39	BD	A	rtM204V + rtL180M/sI195M	-	sI195M - Impair anti-HBs binding [7]
40	BD	A	rtT128N*/sP120T	-	sP120T – Vaccine/HBIg therapy escape [12]
55	BD	A	-	rtL80F, sY100S*	-
58	BD	A	-	sY100C*	-
62	BD	D	rtS219A/sS210R	-	-
64	BD	A	-	sI/T126P, sQ129P, rtT128P, sY100C	sI/T126P and sQ129P- Diagnostic failure [13]
65	BD	A	-	sY100C*	-
69	BD	A		rtL80V , rtT128S, sY100F	-
74	BD	A	-	sY100C*	-
75	BD	F	rtV207L/sM198I	sF183C** , rtL82V*	sM198I - Reduced antigenicity of HBsAg [7]
75	BD	F	rtV207L/sM198I	sF183C** , rtL82V*	sF183C**- Reduced anti-HBs binding [9]
77	BD	A	-	sY100C*	-
521	UP	A	-	sY100C*	-
1150	UP	D	rtS219A/sS210R	-	-

Mutation in bold: Primary mutation related to Lamivudine resistance; Mutations in italic: Compensatory mutations; Underlined Mutations: Have clinical significance; *No in vitro assays; ** Natural polymorphism in the genotype F; ID: Sample identification; BD: Blood Donor; UP: Untreated Patient; HBsAg- Hepatitis B Surface Antigen; POL: Polymerase gene; S: Surface gene; HBIg- Immunoglobulin against Hepatitis B Virus.

ISSN: 1743-422X