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Table 2 Oligonucleotide primers used for RT-LAMP assay and semi-nested RT-PCR against Akabane virus

From: Rapid detection of Akabane virus by a novel reverse transcription loop-mediated isothermal amplification assay (RT-LAMP)

Primers

Sequence (5'-3')

Nucleotide positions*

Length (bp)

F3

TGTTCCACAACGGAATGC

27–44

18

B3

TGTAAATTTGACCCCTGCA

198–216

19

FIP

GAGCTGCTGCCCATACTTACT-AGCTACATTTAATCCGGATGC

 

42

BIP

TACTGTTGCTAGAGTCTTCTTCCTC-AGTAAGATCGACACTTGGTT

 

45

N1

ATGTTCCACAACGGAATGCAGC

25–46

22

N2

AGCCAGGAAAGCTCTAGCTGCAG

680–658

23

N3

GGAAAATGGTTATTAACCACT

215–235

21

  1. *Nucleotide sequence position according to nucleoprotein (N) gene of Akabane virus strain 93FMX (GenBank accession number FJ498797).
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Virology Journal

ISSN: 1743-422X