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Figure 1 | Virology Journal

Figure 1

From: Classical swine fever virus triggers RIG-I and MDA5-dependent signaling pathway to IRF-3 and NF-κB activation to promote secretion of interferon and inflammatory cytokines in porcine alveolar macrophages

Figure 1

Expression of MDA5, RIG-I and IPS-1 in CSFV-infected porcine alveolar macrophages. CSFV Shimen isolates at MOI of 0, 0.1, 0.5, 1 or 3 were used to infect PAMs. Cells treated with poly (I:C) were used as a positive control. At 24 hpi, extracts of circa 20 μg total cell were prepared and subjected to Western Blotting for measuring protein expression of MDA-5 (A), RIG-I (B) or IPS-1 (C), respectively. Anti-β-actin was served as an internal control. The experiment was repeated three times and the figure here shows a representative experiment. An asterisk indicates a statistically significant difference from uninfected cells, * P < 0.05 and ** P < 0.01.

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Virology Journal

ISSN: 1743-422X