Delayed seroconversion to STLV-1 infection is associated with mutations in the pol and rex genes

Table 1 Chronology of serological (ELISA & WB) and PCR analyses of monkeys experimentally infected with STLV-1 Tan 90 or STLV-1 Pat 74

Target monkey	SIV	Inoculumn	Months post exposure	ELISA	WB
Target monkey	SIV	Inoculumn	Months post exposure	ELISA	Status *	rgp21	p19	p24	p26	p28	p32	p36	gp46	p55	rgp46	PCR^▲
Tan 95	+	STLV-1 Tan 90	0	-	-	-	-	-	-	-	-	-	-	-	-	-
			2	-	-	-	-	-	-	-	-	-	-	-	-	-
			4	-	-	-	-	-	-	-	-	-	-	-	-	+
			11	-	I	-	++	-	+	+	-	+	-	-	-	+
			12	+	I	+	++	-	+	+	+	+	-	+	+++	+
			24	+	I	+	++	-	+	+	+	+	-	+	+++	+
Tan 97	-	STLV-1 Tan 90	0	-	-	-	-	-	-	-	-	-	-	-	-	-
			2	-	-	-	-	-	-	-	-	-	-	-	-	-
			4	-	-	-	-	-	-	-	-	-	-	-	-	+
			11	-	+	+	+++	+++	++	+++	+	++	+	+	+	+
			12	+	+	+++	++++	++++	++++	++++	++++	++++	+	+	+++	+
			24	+	+	+++	++++	++++	++++	++++	++++	++++	+	+	+++	+
Pat 73	-	STLV-1 Pat 74	0	-	-	-	-	-	-	-	-	-	-	-	-	-
			2	+	+	+	+++	++	+++	++	++	++	+	+	+	+
			4	+	+	+	+++	++	++++	++++	++++	++++	+	+	+++	+
			5	+	+	++	++++	++++	++++	++++	++++	++++	+	+	+++	+
			16	+	+	++	++++	++++	++++	++++	++++	++++	+	+	++++	+
Patt	-	STLV-1 Pat 74	0	-	-	-	-	-	-	-	-	-	-	-	-	-
			2	+	+	+	+++	+	++	+++	++	++	+	+	+	+
			4	+	+	+++	++++	++++	++++	++++	++++	++++	+	+	+++	+
			5	+	+	+++	++++	++++	++++	++++	++++	++++	+	+	+++	+
			16	+	+	+++	++++	++++	++++	++++	++++	++++	+	+	++++	+

* WB status = + if reactivity to both Gag p24 and Env gp46 original were observed, I + indeterminate, and – if no reactivity at all. The intensity of the WB was rated as ranging from negative (-) to 4 + .
▲ PCR was performed for both pol and pX genes. All + samples were + for both, and all – samples were – for both.

ISSN: 1743-422X