Figure 6
From: A baculovirus-mediated strategy for full-length plant virus coat protein expression and purification
Dot-ELISA of garlic leaf extracts. A shows the antigen (baculovirus-expressed GarMbFV-CP) used to obtain the antiserum, (C+) shows a positive control derived from a GarMbFV-infected leaf extract confirmed by RT-PCR (not shown), in (C-) a virus-free garlic leaf extract as negative control. Nine extracts (1 to 9) from different plants showing virus infection symptoms were denaturated with modified Laemmili’s buffer, manually dotted, and tested with the antiserum obtained in this work. Only sample 4 did not react with the antiserum produced.