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Figure 3 | Virology Journal

Figure 3

From: Porcine epidemic diarrhea virus E protein causes endoplasmic reticulum stress and up-regulates interleukin-8 expression

Figure 3

Effect on porcine GRP78 expression by PEDV E expression in cultured IEC cells. (A) The level of GRP78 expression was determined by western blot with the mouse monoclonal antibodies against GRP78 in all cell lines. β-actin was used as an internal loading control. (B) Total RNA was extracted from cells expressing either GFP alone, GFP-E or untransfected cells. Real-time PCR analysis of GRP78 mRNA levels were normalized to the corresponding CT value for porcine β-actin mRNA. The results are means ± SD and representative of three independent experiments.

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Virology Journal

ISSN: 1743-422X