Figure 3
Phylogenetic identification of OnuMV1c and OnuMV7. The fungal mitovirus RdRp sequences were obtained from the NCBI gene bank database. Multiple sequence alignments were performed using MUSCLE and the tree constructed using NJ method with 1000 bootstrap replicates. Bootstrap values are shown. The WAG substitution model was selected assuming an estimated proportion of invariant sites (of 0.025) and 4 gamma-distributed rate categories to account for rate heterogeneity across sites. Saccharomyces 23S RNA narnavirus [UniProt: Q07048] = RdRp Saccharomyces 23 S RNA narnavirus PE = 1 SV = 2 served as an outgroup. Virus notations were according to Figure 2 and as follows: Sclerotinia sclerotiorum MV2 [GenBank: AEX91879.1] = S. sclerotiorum mitovirus 2; Helicobasidium mompa MV1-18 [GenBank: BAD72871.1] = H. mompa mitovirus 1 – 18; Gremmeniella abietina GMV2 [GenBank: AEY76153.1] = G. abietina non-host-specific mitrochondrial RNA virus S1; Gremmeniella abietina GMV1 [GenBank: CCD32685.2] = RdRp Gremmeniella mitovirus; Sclerotinia sclerotiorum MV1 [GenBank: AEX91878.1] = S. sclerotiorum mitovirus 1; Thielaviopsis basicola MV01 [NCBI Reference Sequence: YP_002822229.1] = RdRp T. basicola mitovirus; Thielavopsis basicola MV02 [GenBank: AAZ99833.1] = RdRp T. basicola mitovirus; Sclerotinia sclerotiorum MV4 [GenBank: AGC24233.1] = RdRp S. sclerotiorum mitovirus 4; Glomus sp. Small MV [GenBank: BAJ23143.1] = Putative RdRp Glomus sp. RF1 small virus; Tuber excavatum MV [GenBank: AEP83726.1] = RdRp T. excavatum mitovirus; Cryphonectria parasitica MV1-NB631 [NCBI Reference Sequence: NP_660174.1] = RdRp C. parasitica mitovirus 1-NB631; Thanatephorus cucumeris MV [GenBank: AAD17381.1] = dsRNA viral RdRp T. cucumeris; Tuber aestivum MV [NCBI Reference Sequence: YP_004564622.1] = RdRp T. aestivum mitovirus; Clitocybe odora MV [NCBI Reference Sequence: YP_005352912.1] = RdRp C. odora virus; Botrytis cinerea dr MV [NCBI Reference Sequence: YP_002284334.1] = RdRp B. cinerea debilitation-related virus; Botrytis cinerea MV1 [GenBank: ABQ65153.3] = RdRp B. cinerea mitovirus 1; Sclerotinia sclerotiorum MV3 [GenBank: AGC24232.1] = RdRp S. sclerotiorum mitovirus 3; Sclerotinia homoeocarpa MV [GenBank: AAO21337.1] = S. homoeocarpa mitovirus.