Figure 4

Effect of F-actin disruption and stabilization on viral protein distribution. MDCK cells were infected with MV at an MOI of 10. To prevent fusion, an inhibitory peptide (FIP) was added. Inhibitors (CD, Jaspla) were added at 12 h p.i.. ( A ) For N and M protein co-staining, cells were fixed and permeabilized with methanol/acetone. N was detected by a polyclonal rabbit antiserum and AF488-conjugated secondary antibodies. M was stained using a monoclonal antibody (MAB8910) and AF568-conjugated secondary antibodies. ( B ) For H and M co-staining, cells were fixed with PFA, permeabilized with Triton X-100 and H was detected with a monoclonal antibody (K83) and AF488-conjugated secondary antibodies. Afterwards, the M protein was stained with an AF555-labelled monoclonal anti-M antibody (MAB8910). xy sections of the merged images (merge xy) and a side view (xz) are shown. Images were recorded with a confocal laser scanning microscope (Zeiss LSM510). Magnification 630x.