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Figure 2 | Virology Journal

Figure 2

From: MicroRNA-145 regulates oncolytic herpes simplex virus-1 for selective killing of human non-small cell lung cancer cells

Figure 2

Expression levels of ICP27 in normal cells and non-small cell lung cancer cells after infection with AP27i145. The mRNA and protein expression levels of ICP27 in AP27i145-infected cells was determined using quantitative reverse transcription polymerase chain reaction assay and Western blot assays. (a) The expression of ICP27 mRNA in AP27i145-infected cells. The mRNA expression level of ICP27 was normalized to an internal control (actin) to obtain ΔCT values, and then all ΔCT values were compared with PL1 cells to give -ΔΔCT. (b) The expression of ICP27 protein in AP27i145-infected cells. The protein expressions of ICP27 in normal cells and non-small cell lung cancer cells after infection with AP27i145 were examined by Western blotting with antibodies specifically against ICP27 and β-actin.

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Virology Journal

ISSN: 1743-422X