Table 2 Primers for PCR amplification of IRES-survival gene cDNAs
From: Engineering lentiviral vectors for modulation of dendritic cell apoptotic pathways
Sequence | Primer direction | Primer sequence |
|---|---|---|
IRES-c-FLIPS | Forward | 5’- ATAACTCGAGTGCCCCTCTCCCTCCCCCC - 3’ |
Reverse | 5’- ATAACTCGAGTGCGGCCGCTCATGCTGGTA - 3’ | |
IRES-c-FLIPL | Forward | 5’- ATAACTCGAGTGCCCCTCTCCCTCCCCCC - 3’ |
Reverse | 5’- ATAACTCGAGTGCGGCCGCTCACGTAGGAG - 3’ | |
IRES-Bcl-XL | Forward | 5’- ATAACTCGAGTGCCCCTCTCCCTCCCCCC - 3’ |
Reverse | 5’- ATAACTCGAGTGCGGCCGCTCACTTCCGA - 3’ | |
IRES-M11L | Forward | 5’- GTCGACGCCCCTCTCCCTCCCCCC - 3’ |
Reverse | 5’- GCGGCCGCCTAGGTCCCTCGGTACCATTTTAGTATTCC - 3’ | |
IRES-AKT-1 | Forward | 5’- GTCGACGCCCCTCTCCCTCCCCCC - 3’ |
Reverse | 5’- GTCGACGCGGCCGCTCAGGCTGT - 3’ |