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Figure 1 | Virology Journal

Figure 1

From: Simultaneous detection and differentiation of Rice black streaked dwarf virus (RBSDV) and Southern rice black streaked dwarf virus (SRBSDV) by duplex real time RT-PCR

Figure 1

Standard curve for TaqMan real time RT-PCR amplification of standard ssRNA (viral transcripts). (A) Amplification plots showing the testing in triplicate of a 10-fold dilution series containing standard ssRNA of RBSDV at: 1x105(a); 1x104(b); 1x103 (c); 1x102(d); 1x101(e) template copies per reaction. (B) Amplification plots showing the testing in triplicate of a 10-fold dilution series containing standard ssRNA of SRBSDV at: 1x106(a); 1x105(b); 1x104 (c); 1x103(d); 1x102(e) template copies per reaction. (C) Amplification plots showing the testing in triplicate of a 10-fold dilution series containing standard ssRNA of eEF-1a at: 1x106(a); 1x105(b); 1x104 (c); 1x103(d); 1x102(e) template copies per reaction. (D) Amplification plots showing the testing in triplicate of a 10-fold dilution series containing standard ssRNA of UBQ5 at: 1x106(a); 1x105(b); 1x104 (c); 1x103(d); 1x102(e) template copies per reaction.

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Virology Journal

ISSN: 1743-422X