Figure 2

Optimum of VRP production. (A) In vitro-synthesized Gluc replicon RNA was coelectroporated with in vitro-synthesized helper-C and helper-E RNA and cells were either incubated at 32°C or 37°C. Supernatants were harvested at the indicated time points after electroporation and Gluc activity was measured in RLUs. (B) Same volumes of supernatants harvested at different time points from the different incubation temperatures in (A) were used to infect fresh BHK cells at 37°C. At 6 h post infection, Gluc activity was measured in the supernatant.