Figure 4

The effect of DING proteins on HIV-1 production in microglial cells. A) Microglial cells were transfected with the pNL43 HIV-1 ∆env pseudovirus genome (30 ng) and DING expression vectors (935 ng). The cell lysates were assayed for luciferase activity 48 hours post-transfection. PA14DING reduced HIV-1 replication by ~40% (p < 0.05). PfluDING did not have a significant effect. This graph is representative of at least three separate experiments. Error bars represent standard error. RLU: relative light units. B) Microglial cells were transfected with the full genome of wildtype HIV-1 strain pNL43 (30 ng) and PA14DING (970 ng). Viral production was determined by measuring levels of the viral capsid protein p24 in the conditioned medium by ELISA 48 hours post-transfection. Serial dilutions of recombinant p24 were used to plot a standard curve which was used to determine the absolute amount of p24 in the conditioned media. PA14DING inhibited HIV-1 replication by ~43% (p < 0.05). The graph is representative of two separate experiments. Error bars represent standard error.