Figure 3
From: Pseudomonas DING proteins as human transcriptional regulators and HIV-1 antagonists
Dose response of PfluDING and PA14DING on Tat-mediated HIV-1 transcription in microglial cells. Microglial cells were transfected with HIV-1 LTR-luc (280 ng), renilla luciferase (100 ng), Tat (13 ng) and increasing amounts of the PfluDING or PA14DING expression vectors (0–500 ng). The total amount of transfected DNA was kept constant at 1 μg by the addition of the empty pcDNA3.2/V5-DEST vector. The cell lysates were assayed for luciferase activities 48 hours post-transfection. A) PA14DING inhibited Tat-mediated HIV-1 transcription in a dose-dependent manner. B) PfluDING had no effect regardless of the dose. The graphs are representative of at least three separate experiments. Error bars represent standard error. RLU: relative light units.