Figure 1
From: Pseudomonas DING proteins as human transcriptional regulators and HIV-1 antagonists
DING proteins elevate NFκB mediated transcription. Plasmids expressing DING and PstS proteins were transfected into HEK 293T cells along with a reporter plasmid containing: A) luciferase downstream of a promoter containing two NFκB binding sites (pBIIXLuc); B) a IL-8 reporter containing the IL-8 gene promoter; C) pBIIXLuc with and without the IκB super-repressor expressing plasmid. The data were normalised with a third plasmid containing Renilla luciferase under the control of a thymidine kinase promoter. The graphs are representative of at least 5 experiments with each protein. RLU: relative light units. D) Western blots showing the expression of V5-tagged (i) PstS, (ii) DING proteins and β-actin controls in transfected HEK 293T cells.