The N-end rule and retroviral infection: no effect on integrase

Table 1 HIV-1 proteins have N-terminal destabilizing residues

HIV-1 cleavage site and conservation	Amino acid at the N terminus	N-end rule designation
MA/CA Conserved	Proline	Stabilizing
CA/p2 Conserved	Alanine	Stabilizing
P2/NC Variable	Methionine	Stabilizing
NC/p1 Conserved	Phenylalanine	Primary Destabilizing
p1/p6(gag) Variable	Leucine	Primary Destabilizing
NC/TFP Conserved	Phenylalanine	Primary Destabilizing
TFP/p6(pol) Variable	Leucine	Primary Destabilizing
p6(pol)/PR Variable	Proline	Stabilizing
PR/RT Conserved	Proline	Stabilizing
RT/RH(p15) Conserved	Tyrosine	Primary Destabilizing
RH(p15)/INT Conserved	Phenylalanine	Primary Destabilizing
Nef Variable	Leucine	Primary Destabilizing

Comparison of 84 protease cleavage site sequences (27 C-type, 30 B-type and 27 M-type) [23]. The position and the conservation relatives to the most common recent common ancestor (MRCA) are shown. The proteins that are conserved for a destabilizing residue as confirmed by an analysis of 1850 isolates of HIV-1 and SIVcpz present in Los Alamos HIV Database (http://www.hiv.lanl.gov) are highlighted in bold.

ISSN: 1743-422X