Artificial microRNA-mediated resistance against the monopartite begomovirus Cotton leaf curl Burewala virus

Table 5 List of oligonucleotides primers used in the study

Primer	Sequence (5′-3′)	Virus/ Construct	Size of amplicon (bp)	Amplicon
BAF	ACGCGTGCCGTGTTGCTGCCCCATTGTCC	begomovirus	~2800	genome
BAR	ACGCGTATGGGCTGYCGAAGTTSASACG
BV2F	ATGTGGGATCCACTGTTAAATGAG	CLCuBuV	356	V2 gene
BV2R	TAGGAACATCTGGACTTCTGTAC
CLCKV2F	GTCGACAAGTATGCGTTTGAAAAATGTG	CLCuKoV	370	V2 gene
CLCKV2R	GGATCCACCTTCACATCCTCTAGGAAC
IRVF	CGTGGAATTCATGTGGGATCCACTGTT	CLCuBuV	348	V2 gene
IRVR	TTCGTCGACGAACATCTGGACTTCTGTA
PedLCV2F	ATGTGGGATCCGTTATTGAAC	PedLCuV	357	V2 gene
PedLCV2F	CTAGGAACATCTGGACTTCTG
TLCNDV2F	GTCGACAAACATGTGGGATCCATTATTGC	ToLCNDV	361	AV2 gene
TLCNDV2R	CCCGGGCTTCTATACATTCTGTAC
P1CF	GACAGTGGTCCCAAAGATGGA	P1C	273	expression cassette*
P1CR	ATCGGGAATAATGGTCCTAA
P1DF	GACAGTGGTCCCAAAGATGGA	P1D	275	expression cassette*
P1DR	TAGCCCATATGCCCGGATTG
35SF	GACAGTGGTCCCAAAGATGGA	pJIT163	150	CaMV 35S promoter
35SR	CAGTGGAGATATCACATCAATCCA
TLCNDBF	GTCCCGGGTATGTCACGTATCCATCA	ToLCNDV DNA B	550	NSP gene
TLCNDBR	TATCGATTTATCCAATGTAATTAAGAAT

* These primer pairs amplify from the 3′ end of the CaMV 35S promoter (P1CF and P1DF) to the 3′ end of the amiRNA construct (primers P1CR and P1DR being the reverse complement of the sequence of the amiRNA backbone [amiRNA* sequence]).

ISSN: 1743-422X