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Figure 3 | Virology Journal

Figure 3

From: Artificial microRNA-mediated resistance against the monopartite begomovirus Cotton leaf curl Burewala virus

Figure 3

Southern blot detection of begomoviruses in inoculated N. benthamiana plants. Blots were probed for the presence of CLCuBuV ( A ), CLCuKoV ( B ), PedLCuV ( C ) or ToLCNDV ( D ). For each blot DNA samples were extracted from inoculated transgenic plant harbouring the P1C (lanes 1 and 2) or P1D (lanes 3 and 4) constructs or inoculated non-transgenic plants (lanes 6 and 7; note DNA from only a single plant was used in panel A). DNA extracted from a non-inoculated transgenic plant was run in lane 5. Approx. 10 μg of total DNA was loaded in each case. The positions of viral single-stranded (ss), super-coiled (sc), linear (lin) and open-circular (oc) DNA forms are indicated.

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Virology Journal

ISSN: 1743-422X