Skip to main content
Figure 6 | Virology Journal

Figure 6

From: Active vaccination with vaccinia virus A33 protects mice against lethal vaccinia and ectromelia viruses but not against cowpoxvirus; elucidation of the specific adaptive immune response

Figure 6

Expression and induction of antibodies with comet inhibition activity by Sindbis A33 harboring point mutations within the CP II region. Multiple sequence alignment of A33 CP II region showing amino acid changes between several Orthopoxviruses-VACV-WR (Vaccinia virus – Western Reserve), CPXV-BR (CPXV Brighton red), MPXV-Zar (monkeypox Zaire), ECTV-Moscow and VARV-BGD74-sol (Bangladesh 74) (A). Expression analysis of the various A33 genes in lysates of Sindbis A33 infected BHK21 cells separated by SDS-PAGE and immunoblotted as indicated. MW – Molecular weight (Kda). Arrowheads indicate bands of the different proteins (B). Geometric mean titer (GMT) (ELISA) in sera of vaccinated mice 21 days post vaccination. Bars represent GeoStDev (C). Comet inhibition activity in sera of Sindbis A33 vaccinated mice (D, same sera as in C). Serum dilutions are indicated to the left and the type of Sindbis A33 used for vaccination is indicated at the top.

Back to article page

Virology Journal

ISSN: 1743-422X