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Figure 5 | Virology Journal

Figure 5

From: Active vaccination with vaccinia virus A33 protects mice against lethal vaccinia and ectromelia viruses but not against cowpoxvirus; elucidation of the specific adaptive immune response

Figure 5

Mapping the protective epitope to the CP II region in A33. Alignment of the amino-acid sequence (aa 73–137) of A33VACVof: VACV-WR (Vaccinia virus – Western Reserve) with the ortholog A33 of CPXV-BR (CPXV Brighton red), MPXV-Zar (monkeypox Zaire), ECTV-Moscow and VARV-BGD74-sol (Bangladesh 74). CP I and CP II represent two regions with variable changes between the sequences (A). BALB/c mice were vaccinated with 1E + 7 pfu Sindbis viruses expressing A33VACV, A33CPXV or with A33VACV in which the CP I or CP II regions from A33CPXV were introduced into its genome replacing the original regions (domain swapping). Two weeks later, the mice were challenged with either VACV-WR (4E + 5 pfu corresponding to 20 i.n. LD50) (B) or with CPXV Brighton-red (3E + 5 pfu corresponding to 3 i.n. LD50) (C) (n = 5 per group). Error bars represent standard errors of the mean (SEM).

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Virology Journal

ISSN: 1743-422X