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Figure 1 | Virology Journal

Figure 1

From: Construction and characterization of an infectious replication competent clone of porcine endogenous retrovirus from Chinese miniature pigs

Figure 1

RT-PCR and PCR analysis of RNA or DNA from the WZS-PERV(2) transfected HEK293 cell or the HEK293 cells infected with PERV derived from WZS-PERV(2). A. Screening of infectious clones with RT-PCR: Lane M DL 2000 DNA Marker; lane a PCR products of genomic DNA from PK15 cells; lane g PCR products of genomic DNA from fresh HEK293 cells; lane h blank control; lane b-f RT-PCR products of RNA from the HEK293 cells transfected with 1–5 recombinant plasmids. B. PCR products of genomic DNA from the infected cells at 72 hours after infection: lane a PCR products of genomic DNA from PK15 cells; lane b PCR products of genomic DNA from the infected HEK293 cells; lane c PCR products of genomic DNA from fresh HEK293 cells.

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Virology Journal

ISSN: 1743-422X