Skip to main content
Figure 6 | Virology Journal

Figure 6

From: Inhibition of hepatitis B virus (HBV) gene expression and replication by HBx gene silencing in a hydrodynamic injection mouse model with a new clone of HBV genotype B

Figure 6

The effect of HBxi285 on HBV gene expression and replication in vivo. 10 μg pAAV-HBV1.3B and 10 μg pSB-HBxi285, pSR-HBxi285 or control plasmids were co-injected into the tail veins of C57BL/6 mice. Each group included 10 mice. (A) HBsAg levels, (B) HBV DNA and (C) the positive rate of HBsAg in the serum of C57BL/6 mice treated with pAAV-HBV1.3B and pSR-HBxi285 or pSB-HBxi285. Value 0 of HBV DNA means the copy number of HBV DNA is lower than detection limit. The data were analyzed by one-way ANOVA, and the differences were statistically significant (# : p < 0.05 pSR-HBxi285 group vs. pSR-HBxicon group; * : p < 0.05 pSR-HBxi285 group vs. pSB-HBxi285 group by one-way ANOVA) (D) Immunohistochemical staining for HBcAg in hepatocytes of C57BL/6 mice treated with pSR-HBxi285, pSB-HBxi285, pSR-HBxicon or pSR vector separately at 3 day post injection (1–4) and C57BL/6 mice treated with pSR-HBxi285 or pSR-HBxicon in the 4th wpi (5–6) (Original magnification: 200X).

Back to article page

Virology Journal

ISSN: 1743-422X