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Figure 8 | Virology Journal

Figure 8

From: Isolation and genetic characterization of human coronavirus NL63 in primary human renal proximal tubular epithelial cells obtained from a commercial supplier, and confirmation of its replication in two different types of human primary kidney cells

Figure 8

Cytopathic effects formed by HCoV-NL63/RPTEC/2004 pp A and by HCoV-NL63 Amsterdam −1. [A]. Non-infected LLC-MK2 cells, 8 days, 33°C (400 X). [B] LLC-MK2 cells infected with HCoV-NL63/RPTEC/2004 pp A, 8 days p.i., 33°C, showing a syncytium, rounding of some cells, and areas of clearing (400X). [C] Non-infected HEK-293 cells, 8 days, 37°C (400X). [D] Advanced cytopathic effects in HEK-293 cells 8 days p.i. with HCoV-NL63/RPTEC/2004 ppA, 37°C (400X). [E] LLC-MK2 cells infected with HCoV-NL63/Amsterdam-1, 33°C, 6 days p.i; detached cells, areas of clearing, vacuolation, and a small syncytium are visible (400X). [F] Vero cells infected with HCoV-NL63/Amsterdam-1, 33°C, 8 days p.i.. Vacuolation, a few floating dead cells, large areas of clearing, and a small syncytium are visible (400X).

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