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Figure 12 | Virology Journal

Figure 12

From: Isolation and genetic characterization of human coronavirus NL63 in primary human renal proximal tubular epithelial cells obtained from a commercial supplier, and confirmation of its replication in two different types of human primary kidney cells

Figure 12

Virus titers three days post-infection of primary cells infected with HCoV-NL63/RPTEC/2004 pp A or HCoV-NL63/Amsterdam-1. Titers were obtained from free virus in spent media; plaque assays were performed in CaCo-2 cells. Average virus titers for HCoV-NL63/RPTEC/2004 pp A (PFU/ml, mean of 3 measurements) were: RPTEC, 6.9 × 107; HRE cells, 6.4 × 107; HRCE cells, 5.2 × 103. Average virus titers for HCoV-NL63/Amsterdam-1 (PFU/ml, mean of 3 measurements) were: RPTEC, 6.9 × 107; HRE cells, 7.8 × 107; HRCE cells, 5.1 × 103.

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