Maintaining the structural integrity of the bamboo mosaic virus3′ untranslated region is necessary for retaining the catalytic constant for minus-strand RNA synthesis

Table 1 Kinetic parameters of the in vitro BaMV RNA replication for the short transcript templates

	rABCDE^a	rABCP	rP	rDE
^bK_M (nM)	302^e	296	533	209
^cV_max (min^-1) (rel V_max)	63 (1.00)	21 (0.33)	25 (0.40)	17 (0.25)
^dV_max/K_M (mM^-1s^-1) (rel V_max/K_M)	3.477 (1.00)	1.182 (0.34)	0.782 (0.22)	1.356 (0.34)

^aThe short transcripts used in the in vitro BaMV RNA replication are indicated in Figure 1.
^bApparent K_M is defined as the concentration of RNA molecules that permits half-maximal rate of reaction. It stands for how effectively the enzyme would bind the substrate.
^cV_max is the maxim initial velocity that an enzyme can achieve. It stands for the rate at which a substrate will be converted to product once bound to the enzyme. The relative activity is shown in the bracket.
^dV_max/K_M stands for the overall specificity of the enzyme reaction.
^eThe parameters were calculated by creating a Lineweaver-Burk plot derived from the Michaelis-Menten equation (Figure 5).

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