A novel dengue virus detection method that couples DNAzyme and gold nanoparticle approaches

Table 2 Nucleotide sequences of active and negative control DNAzymes and corresponding targets

DNAzyme	5’Arm (5’-- > 3’)	3’Arm (5’-- > 3’)	Catalytic core	Target
DDZ-M	TTTCTCTCG	GTTTCAGCA	GGCTAGCTACAACGA	TGCTGAAACGCGAGAGAAA
DDZ-1	ATCGCTCCA	TCTTCTTGA	GGCTAGCTACAACGA	TCAAGAAGAATGGAGCGAT
DDZ-2	AAAGGCGTA	TTCTCGCCT	GGCTAGCTACAACGA	AGGCGAGAAATACGCCTTT
DDZ-3	TAGCCAAGA	TCCTGCTGT	GGCTAGCTACAACGA	ACAGCAGGAGTCTTGGCTA
DDZ-4	GTTGGTTCA	TTTTCCAGA	GGCTAGCTACAACGA	TCTGGAAAAATGAACCAAC
Negative Control
DDZin-M	TTTCTCTCG	GTTTCAGCA	AGCAACATCGATCGG	TGCTGAAACGCGAGAAA

Left column lists the active (DDZ-M) and inactive (DDZin-M) DNAzymes used in this report. The second and thrid columns list the sequences of the 5’and 3’ binding arms of the catalytically active DNAzymes and the inactive DDZin-M, respectively. Also shown are the sequences of the catalytic cores of each DNAzyme. The right column lists the nucleotide sequence each binding arm binds to where applicable. All sequences are displayed in a 5’ to 3’ direction. See Methods for description of DNAzyme design.

ISSN: 1743-422X