Figure 2
From: A novel dengue virus detection method that couples DNAzyme and gold nanoparticle approaches
Anatomy of the 10-23 anti-DENV DNAzyme (DDZ) and Schematic Diagram of the DENV genome. A) DDZ was designed and produced as previously described [36, 85] and in Materials and Methods. R = Purine. Y = Pyrimidine. The target RNA is in green with the anti DENV DNAzyme 10-23 shown in black. The 5’ and 3’ ends of target RNA and DNAzymes are as indicated. Thin black vertical lines show complimentary base pairing. Arrows indicate cleavage site of the target RNA. B) A representation of the 10,723 base DENV-2 NGC GC capped and polyadenylated genome is shown to illustrate the position of the region targeted by the anti-DENV DNAzyme, DDZ. Non structural (NS) genes 1 through 5 are shown in green. Structural genes encoding capsid, membrane glycoprotein precursor, and envelope proteins are shown in red. UTR = untranslated region, PA = polyadenylation.