Figure 4
(A) Virus titers, (B) weight loss and (C) mortality of 10LD 50 instilled PR8. Groups of mice (n = 10) were immunized as previously and challenged by instillation of PR8. Four days after challenge, 5 mice in each group were euthanized to allow titration of virus in the lungs, the weight and survival of the remaining mice was monitored until day 12. (A) shows geometric mean virus titers, together with the standard deviation; the dashed line is the limit of quantification for this assay. (B) shows the percent reduction in weight relative to the baseline body weights, with error bars showing standard deviation. A key for the groups is shown to the right of this graph. (C) shows percent survival of mice in each group. A key for these groups is shown to the right of the graph. Survival of mice vaccinated with Ad-HA and Ad-M2 was significantly greater (Mantel-Cox test, p = 0.0016) than mice immunized with Ad-NPA or Ad-NPB. The latter 2 groups were not different from one another or naïve mice.