Figure 7

Correlation between qPCR-MN and plaque-reduction neutralization ( PRN). Neutralization assays were performed in the (A) absence or (B) presence of 5% guinea pig complement with RSV-A2 using a panel of human serum samples (n = 30). PRN was performed using HEp-2 cells (50% inhibition endpoint). qPCR-MN (90% inhibition endpoint; geometric mean titers calculated from two experimental replicates) was performed using Vero cells (500 TCID₅₀ per well of virus input; assessment at 24 hours post-infection).