Figure 2

Replication kinetics of RSV. Vero cells were seeded in 96-well culture plates (15,000 cells per well). On the following day, cells were infected with RSV-A2 or RSV-B1 (500 TCID₅₀ per well). After incubation for 1 hour at 37°C, the cells were washed twice with PBS to remove unbound virus; subsequently, the culture medium was replaced and the infection was allowed to proceed at 37°C. At the indicated times, cell lysates were prepared using Bio-Rad SPR and subjected to one-step SYBR Green qRT-PCR. RNA copy numbers were normalized to the mean value obtained at 24 hours post-infection for each virus strain. Each point represents the mean with corresponding range (n = 3).