Figure 1

SYBR Green qRT-PCR targeting the RSV N gene. Total RNA was purified from Vero cells infected with either RSV-A2 or RSV-B1. The resulting RNA standards were serially diluted (10-fold); a cell lysate, prepared from uninfected Vero cells with the Bio-Rad iScript Sample Preparation Reagent (Bio-Rad SPR), was used as the diluent (the initial dilution contained ~10 ng/μL of RNA standard). One μL of each dilution was subjected to one-step SYBR Green qRT-PCR (10 μL total reaction volume). Mean threshold cycle (C_t; n = 2) is plotted against log₁₀ (RNA dilution factor) for each RNA standard dilution series: (A) RSV-A2 RNA and (B) RSV-B1 RNA.