LDVICp016 and LDVICp95 are novel members of the vTNFR family. A. Schematic representation of the potential secreted vTNFRs encoded by LCDV-C ORF 16 L indicating complete CRD and signal cleavage site. CRDs were identified searching for the Prosite motif PS50050 (TNFR/NGFR family cysteine-rich region domain profile) online at http://prosite.expasy.org and signal peptide predicted using SignalP 4.0 software (http://www.cbs.dtu.dk/services/SignalP/). B. Sequence alignment of LDVICp016 [Genbank: YP_073525], LDVICp095 [Genbank: YP_073601], Oryzias latipes osteoprotegerin a precursor [Genbank: NP_001239169.1] (Medaka OPG) and Vaccinia virus strain Lister CrmE [Genbank: CAC83048.1]. Conserved positions are indicated with asterisks below the sequence. The position of complete CRDs and predicted signal peptide cleavage site in LDVICp016 are indicated by black lines or arrow, respectively. Sequences were aligned using clustalw online at http://www.ebi.ac.uk/Tools/msa/clustalw2.