Figure 2

Analytical sensitivity of the in-house real-time PCR. Prior to amplification, 10-fold serial dilutions of HAdV-C type 6 were processed by homogenization and heat treatment (open circles, solid line), or nucleic acid extraction (filled squares, dashed line). In both cases, equivalent results were obtained in respect to: A) the linear range; and B) the LoD determined by Probit analysis (n = 24). At a probability of 95%, the LoD for the homogenization- and nucleic acid extraction-based protocols were 12 copies/reaction (log₁₀ = 1.08) and 18 copies/reaction (log₁₀ = 1.08), respectively. The same dilutions used for inoculate virus culture and DFA staining (indicated by open triangles, dotted line) were also quantified and demonstrated a LoD of approximately 380 copies/ml (log₁₀ = 2.58).