A one step real-time RT-PCR assay for the quantitation of Wheat yellow mosaic virus(WYMV)

Table 2 Detection of WYMV from wheat samples by real-time RT-PCR and gel-based RT-PCR

Collection sites	Date	Number of samples	Positive samples by real-time RT-PCR	Positive samples by gel-based RT-PCR	Consistency (%)^a
Zhumadian	2012	10	6	4	4/6 (66.6)
Yangzhou	2012	10	4	2	2/4 (50)
Yantai	2012	10	4	1	1/4 (25)
Nanjing	2011	10	3	1	1/3 (33.3)
Huangchuan	2004	1	1	1	1/1 (100)
Jiangyan	2006	1	1	1	1/1 (100)
Zhouzhi	2007	1	1	1	1/1 (100)
Xiaqiao	2011	1	1	1	1/1 (100)
Total		44	21	12	12/21 (57.1)

^a Results of individual samples diagnosed by real-time RT-PCR and gel-based RT-PCR were compared.

ISSN: 1743-422X