Skip to main content

Table 1 Detection of WYMV in tenfold serial dilutions of the crude sap from infected wheat by indirect ELISA and real-time RT-PCR

From: A one step real-time RT-PCR assay for the quantitation of Wheat yellow mosaic virus(WYMV)

 

Negative controla

Positive controlb

1/10th

1/102th

1/103th

1/104th

1/105th

1/106th

Mean of A405c

0.041

1.093

0.79

0.073

0.052

0.013

0.012

0.008

Number of WYMV genomic RNA copies

–

3.09×107

2.99×106

3.11×105

3.10×104

2.91×103

3.15×102

30

  1. a Healthy wheat leaf; b WYMV-infected wheat leaf; c Mean calculated using all replications.
Back to article page

Virology Journal

ISSN: 1743-422X