In vivo characterization of chimeric PCV DNA clones containing heterogeneous capsid protein nuclear localization signals (NLS)

Table 2 Histopathological lesions in tissues of inoculated and control mice

Group	Inoculum^a	dpi	No. of mice with microscopic lesions/no. tested at dpi^b
			Lung	Lymph node^c	Spleen
1	MEM	7	0/4	0/4 (0.0)^I	0/4 (0.0)^I
		14	0/4	0/4 (0.0)^I	0/4 (0.0)^I
		28	0/4	0/4 (0.0)^I	0/4 (0.0)^I
		42	0/4	0/4 (0.0)^I	0/4 (0.0)^I
2	PCV1 DNA	7	0/4	0/4 (0.0)^I	0/4 (0.0)^I
		14	0/4	0/4 (0.0)^I	0/4 (0.0)^I
		28	0/4	0/4 (0.00)^I	0/4 (0.0)^I
		42	0/4	0/4 (0.00)^I	0/4 (0.0)^I
3	PCV2 DNA	7	0/4	2/4 (0.5)^I	0/4 (0.0)^I
		14	0/4	4/4 (1.0)^II	1/4 (0.25)^I
		28	1/4	4/4 (1.5)^II	2/4 (0.75)^II
		42	0/4	3/4 (1.75)^II	1/4 (0.5)^II
4	PCV12 DNA	7	0/4	0/4 (0.0)^I	0/4 (0.0)^I
		14	0/4	0/4 (0.0)^I	0/4 (0.00)^I
		28	0/4	2/4 (0.5)^I	1/4 (0.25)^I
		42	0/4	2/4 (0.5)^I	1/4 (0.25)^I
5	PCV1-NLS2 DNA	7	0/4	0/4 (0.0)^I	0/4 (0.0)^I
		14	0/4	0/4 (0.0)^I	0/4 (0.0)^I
		28	0/4	0/4 (0.0)^I	0/4 (0.0)^I
		42	0/4	0/4 (0.0)^I	0/4 (0.0)^I
6	PCV2-NLS1 DNA	7	0/4	0/4 (0.0)^I	0/4 (0.0)^I
		14	0/4	0/4 (0.0)^I	0/4 (0.0)^I
		28	0/4	1/4 (0.25)^I	1/4 (0.25)^I
		42	0/4	1/4 (0.25)^I	0/4 (0.0)^I

^aMEM was used as a negative control.
^bFour mice were tested in each group at different time points following inoculation.
^cValues in parentheses are mean scores (0, normal; 1, mild; 2, moderate; 3, severe) for lymphoid depletion and histiocytic infiltration of 4 mice in each group. Different superscripts (^I and ^II) indicate that the mean scores differ significantly between the groups (P < 0.05).

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